L-selectin and leukocyte function in skeletal muscle reperfusion injury.

Publication/Presentation Date

10-1-1999

Abstract

HYPOTHESIS: Treatment with anti-L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle.

DESIGN: Prospective, randomized experimental trials.

SETTING: Basic science research laboratory.

MATERIALS: Male Wistar rats weighing 109 +/- 5 g (mean +/- SEM).

INTERVENTIONS: Gracilis pedicle muscle flaps were elevated and microcirculation was observed by intravital microscopy. Two groups were evaluated: (1) the control group, which received 4 hours of global ischemia, and (2) the experimental group, which received 4 hours of global ischemia, plus treatment with anti-L-selectin monoclonal antibody 30 minutes before reperfusion.

MAIN OUTCOME MEASURES: The number of rolling and adherent leukocytes in postcapillary venules were counted in the 2 groups at baseline and at 1 through 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion.

RESULTS: Treatment with the monoclonal antibody to L-selectin significantly reduced the number of rolling leukocytes (flux) at 2 through 5, 20, 30, 45, and 60 minutes of reperfusion compared with controls (P

CONCLUSION: L-Selectin plays a significant role in leukocyte rolling and adherence to venular endothelium in rat skeletal muscle ischemia reperfusion injury.

Volume

134

Issue

10

First Page

1079

Last Page

1081

ISSN

0004-0010

Disciplines

Medicine and Health Sciences

PubMedID

10522850

Department(s)

Department of Surgery

Document Type

Article

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