L-selectin and leukocyte function in skeletal muscle reperfusion injury.
HYPOTHESIS: Treatment with anti-L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle.
DESIGN: Prospective, randomized experimental trials.
SETTING: Basic science research laboratory.
MATERIALS: Male Wistar rats weighing 109 +/- 5 g (mean +/- SEM).
INTERVENTIONS: Gracilis pedicle muscle flaps were elevated and microcirculation was observed by intravital microscopy. Two groups were evaluated: (1) the control group, which received 4 hours of global ischemia, and (2) the experimental group, which received 4 hours of global ischemia, plus treatment with anti-L-selectin monoclonal antibody 30 minutes before reperfusion.
MAIN OUTCOME MEASURES: The number of rolling and adherent leukocytes in postcapillary venules were counted in the 2 groups at baseline and at 1 through 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion.
RESULTS: Treatment with the monoclonal antibody to L-selectin significantly reduced the number of rolling leukocytes (flux) at 2 through 5, 20, 30, 45, and 60 minutes of reperfusion compared with controls (P
CONCLUSION: L-Selectin plays a significant role in leukocyte rolling and adherence to venular endothelium in rat skeletal muscle ischemia reperfusion injury.
Published In/Presented At
Lozano, D. D., Kahl, E. A., Wong, H. P., Stephenson, L. L., & Zamboni, W. A. (1999). L-selectin and leukocyte function in skeletal muscle reperfusion injury. Archives of surgery (Chicago, Ill. : 1960), 134(10), 1079–1081. https://doi.org/10.1001/archsurg.134.10.1079
Medicine and Health Sciences
Department of Surgery