Mesenchymal commitment to digital joint formation.
Publication/Presentation Date
7-1-1995
Abstract
Temporal and spatial commitment of in vivo and in vitro mammalian digital joint development were characterized in a murine model. Alcian blue and alizarin red staining were used to label proteoglycans of cartilage matrix and mineralized matrix in both whole mounts and histological sections. Mesenchymal differentiation toward a joint fate was identified by a lack of matrix deposition in islands of joint precursor cells between phalangeal precursors, and localized lysosomal enzyme activity was later demonstrated in these regions during formation of the joint cavity. Organ-cultured forelimbs and in vivo specimens demonstrated analogous digital joint morphological trends. With a defined developmental window, reverse transcription, polymerase chain reaction, demonstrated differential gene expression of transforming growth factor-beta isotypes, aggrecan core protein, and type II collagen, suggesting a role for transforming growth factor-beta in directing digital joint development.
Volume
35
Issue
1
First Page
95
Last Page
104
ISSN
0148-7043
Published In/Presented At
Ginsburg, G. T., Royster, D., Kassabian, G., Shuler, C. F., Dougherty, W. R., & Sank, A. C. (1995). Mesenchymal commitment to digital joint formation. Annals of plastic surgery, 35(1), 95–104. https://doi.org/10.1097/00000637-199507000-00019
Disciplines
Medicine and Health Sciences
PubMedID
7574296
Department(s)
Department of Surgery
Document Type
Article