Expression of cyclin protein after thermal skin injury in a guinea pig model.
Publication/Presentation Date
1-1-1997
Abstract
In this study, an immunohistochemical stain for cyclin was used to quantitate proliferating elements in hair follicles at the edge of and within thermal burn areas. Biopsy specimens from thermal injury in the guinea pig (day 1 through day 28) were sectioned and stained with MIB-1 antibody, which recognizes cyclin, a protein expressed during epithelial cell proliferation. At the edge of the burn, 89 +/- 6.1 (SD) cells per medium power field (x 10, mpf) were MIB-1-positive on days 1 through 16. On day 17, the number of positive cells increased, reaching peak values on days 20 to 28 (271 +/- 12.7 cells/mpf). Within the burn, minimal staining was observed from day 1 to day 15 (12.7 +/- 1.6 cells/mpf). Thereafter the number of MIB-1-positive cells increased and plateaued with an average of 96.4 +/- 9.0 cells/mpf on days 20 through 28. In conclusion, immunohistochemical staining of dermal biopsy specimens with MIB-1 antibody may provide a quantitative method for the evaluation of tissue damage and healing after thermal injury.
Volume
18
Issue
4
First Page
292
Last Page
298
ISSN
0273-8481
Published In/Presented At
DeCherney, A. H., Dougherty, W. R., Felix, J. C., Rodgers, K. E., Girgis, W., Abiko, M., & diZerega, G. S. (1997). Expression of cyclin protein after thermal skin injury in a guinea pig model. The Journal of burn care & rehabilitation, 18(4), 292–298. https://doi.org/10.1097/00004630-199707000-00003
Disciplines
Medicine and Health Sciences
PubMedID
9261693
Department(s)
Department of Surgery
Document Type
Article