Dephosphorylation of circulating human osteopontin correlates with severe valvular calcification in patients with calcific aortic valve disease.
Publication/Presentation Date
3-1-2012
Abstract
CONTEXT: Calcific Aortic Valve Disease (CAVD) is an active pathological process leading to biomineralization of the aortic cusps. We characterized circulating and tissue Osteopontin (OPN) as a biomarker for CAVD.
OBJECTIVES: Here we investigate the post-translational modifications of circulating OPN and correlate the phosphorylation status with the ability to prevent calcification.
METHODS: Circulating OPN levels were estimated in CAVD patients (n = 51) and controls (n = 56). In a subgroup of 27 subjects, OPN was purified and the phosphorylation status analyzed.
RESULTS: Plasma OPN levels were significantly elevated in CAVD patients as compared to the controls and correlates with the aortic valve calcium score. Our study demonstrates that phospho-threonine levels of OPN purified from controls were higher when compared to CAVD subjects, whereas phospho-serine and phospho-tyrosine levels were comparable between the two groups.
CONCLUSION: The dephosphorylation of circulating OPN correlates with severe valvular calcification in patients with CAVD.
Volume
17
Issue
2
First Page
111
Last Page
118
ISSN
1366-5804
Published In/Presented At
Sainger, R., Grau, J. B., Poggio, P., Branchetti, E., Bavaria, J. E., Gorman, J. H., 3rd, Gorman, R. C., & Ferrari, G. (2012). Dephosphorylation of circulating human osteopontin correlates with severe valvular calcification in patients with calcific aortic valve disease. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 17(2), 111–118. https://doi.org/10.3109/1354750X.2011.642407
Disciplines
Medicine and Health Sciences
PubMedID
22191734
Department(s)
Department of Surgery
Document Type
Article