Increased lethality of endotoxemia in murine frostbite.
Publication/Presentation Date
10-1-1986
Abstract
Because frostbite (FB) is associated with increased intravascular coagulability, it is reasonable to assume that endotoxin, by enhancing platelet aggregation, will adversely affect FB. Swiss mice (25 +/- 2 g) were anesthetized, and the tails of the animals totally immersed in a freezing solution of equal volumes of ethylene glycol and water (-18 C) for 8 min. The tails were then thawed at room temperature (24 C). Half an hour after removal from the freezing solution, the animals were given either (Group A) 0.1 cc saline I.P. or (Group B) 0.1 mg E. coli endotoxin (055:B5; 1/3 LD50 dose) in 0.1 cc saline IP. A third group (Group C), was given the same dose of endotoxin but was not subjected to frostbite. Survivals in each group at 2 weeks were as follows: (A) 14/14 (100%), (B) 4/20 (20%), (C) 13/14 (93%). Using Fisher's exact test, A versus B P less than .001; B versus C P less than .001; A versus C NS. The data presented here emphasize the increased lethality of endotoxemia in murine FB.
Volume
52
Issue
10
First Page
564
Last Page
567
ISSN
0003-1348
Published In/Presented At
Spillert, C. R., Barillo, D. J., & Lazaro, E. J. (1986). Increased lethality of endotoxemia in murine frostbite. The American surgeon, 52(10), 564–567.
Disciplines
Medicine and Health Sciences
PubMedID
3532892
Department(s)
Department of Surgery
Document Type
Article