Detection of altered H-ras proteins in human tumors using western blot analysis.
Publication/Presentation Date
8-1-1989
Abstract
H-ras p21 protein expression was investigated in bladder and colonic tumor tissues using an H-ras specific antibody in Western blot analysis. The specificity of this antibody to H-ras proteins was established using NIH/3T3 transfectants expressing oncogenic counterparts of the different ras gene family members. Use of this antibody to detect altered H-ras proteins was demonstrated using a panel of transfectants bearing different mutated H-ras genes and established cell lines previously characterized in transfection assays. Extension of this technique to direct analysis of human tumor material confirmed previous observations of H-ras activation within a group of bladder tumors and identified three more urothelial tumors expressing altered H-ras proteins. The altered migrational properties of these three were suggestive of point mutational events in 12 (1 case) and 61 (2 cases) codon hot spots. This study extends previous observations on the preferential activation of H-ras in urinary tract tumors and provides a rapid technique for evaluating the status of H-ras proteins in human tumor tissue.
Volume
61
Issue
2
First Page
212
Last Page
218
ISSN
0023-6837
Published In/Presented At
Joyce, A. D., D'Emilia, J. C., Steele, G., Jr, Libertino, J. A., Silverman, M. L., & Summerhayes, I. C. (1989). Detection of altered H-ras proteins in human tumors using western blot analysis. Laboratory investigation; a journal of technical methods and pathology, 61(2), 212–218.
Disciplines
Medicine and Health Sciences
PubMedID
2666743
Department(s)
Department of Surgery
Document Type
Article