Preimplantation alteration of adenine nucleotides in cryopreserved heart valves.
Publication/Presentation Date
2-1-1993
Abstract
To assess the initial metabolic phase of cellular injury from cardiac valve processing, high-energy phosphate concentrations were analyzed in valve leaflets subsequent to critical processing steps. Using a porcine model, valves were processed in a manner identical to human homografts, with 58 randomly assigned to five groups representing distinct preparation phases. Group I (controls) sustained 40 minutes of warm ischemia concluded by liquid nitrogen immersion. Remaining groups similarly endured 40 minutes of ischemia, but were subsequently prepared according to stepwise design: II, warm ischemia + 24 hours of 4 degrees C ischemia; III, warm ischemia + 24 hours of 4 degrees C antibiotic disinfection; IV, warm ischemia + 24 hours at 4 degrees C (without antibiotics) + cryopreservation (-1 degrees C/min cryoprotected freezing); and V, warm ischemia+disinfection+cryopreservation. At each regimen's conclusion leaflet extracts were assayed by high-performance liquid chromatography for high-energy adenine nucleotides (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate) and catabolites. A 47% and 86% decrease in cellular adenosine triphosphate level was observed in group III and group V leaflets, respectively. The level of total adenine nucleotides was maintained up to cryopreservation; thereafter a 74% decrease was noted. Catabolite analysis confirmed incomplete degradation of adenine nucleotides indicating cellular metabolic resilience throughout standard homograft preparation in valves previously exposed to 40 minutes of warm ischemia.
Volume
55
Issue
2
First Page
413
Last Page
419
ISSN
0003-4975
Published In/Presented At
Domkowski, P. W., Messier, R. H., Jr, Crescenzo, D. G., Aly, H. S., Abd-Elfattah, A. S., Hilbert, S. L., Wallace, R. B., & Hopkins, R. A. (1993). Preimplantation alteration of adenine nucleotides in cryopreserved heart valves. The Annals of thoracic surgery, 55(2), 413–419. https://doi.org/10.1016/0003-4975(93)91013-d
Disciplines
Medicine and Health Sciences
PubMedID
8431053
Department(s)
Department of Surgery
Document Type
Article