Identification of Experimentally Induced Colitis by in Vitro Nuclear Magnetic Resonance.

Publication/Presentation Date

1-1-1985

Abstract

The present study determined whether in vitro nuclear magnetic resonance could be used to assess experimentally induced colitis in rats. Acute colitis was induced in 6 Sprague-Dawley rats by acetic acid enema, while 6 control animals received saline enemas. All animals were sacrificed 24 hours post-enema, and NMR relaxation times, T1 and T2, of colonic samples were determined on a 10 MHz spin analyzer (RADX, Houston, TX). Colonic water content was determined on the same samples by desiccation. Colitis animals showed significantly higher T1 and T2 relaxation times and tissue water content than controls. T1 and T2 times correlated significantly with tissue water content. Twelve additional animals were studied histologically, six of which received acetic acid enemas and showed extensive transmural colitis. Our results suggest that in vivo proton NMR might be a useful means of non-invasively assessing the degree of colonic inflammation.

Volume

17

Issue

2

First Page

197

Last Page

203

ISSN

0748-6642

Disciplines

Other Medical Specialties | Surgery

PubMedID

4080825

Department(s)

Department of Surgery, Department of Surgery Faculty

Document Type

Article

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