Common antigenic sites on exfoliated cells derived from cervical carcinoma and in tumor cells of nonuterine origin as demonstrated by monoclonal antibodies in immunoperoxidase assay.
Publication/Presentation Date
11-1-1985
Abstract
The binding characteristics of monoclonal antibodies produced against a variety of human tumor cells were studied on cervical carcinoma cell lines and on exfoliated cells of cervical smears. The latter included normal epithelial cells, cells derived from cervical intraepithelial neoplasia, and cells from squamous cell carcinoma. Monoclonal antibodies that bound in immunoperoxidase assays to ethanol-fixed smears of cultured human tumor cells but not to normal cervical smears were screened on cervical smears containing malignant cells. Of the six antibodies selected for detailed studies, two each had been produced against bladder carcinoma and melanoma and one each against cervical and gastric carcinoma. Antibody 99-57 stained malignant cells from invasive carcinoma but not normal cervical cells. In cells from intraepithelial neoplasia, staining intensity was highest in severely dysplastic cells. Thus monoclonal antibodies are potentially useful in the detection of malignant cervical cells within a large number of nonmalignant cells, in conjunction with other diagnostic procedures.
Volume
45
Issue
11 Pt 2
First Page
5964
Last Page
5968
ISSN
0008-5472
Published In/Presented At
Koprowska, I., Zipfel, S. A., Himes, T. R., & Herlyn, M. (1985). Common antigenic sites on exfoliated cells derived from cervical carcinoma and in tumor cells of nonuterine origin as demonstrated by monoclonal antibodies in immunoperoxidase assay. Cancer research, 45(11 Pt 2), 5964–5968.
Disciplines
Medicine and Health Sciences
PubMedID
2414003
Department(s)
Department of Medicine
Document Type
Article