Novel pathways in the pathobiology of human abdominal aortic aneurysms.
Publication/Presentation Date
1-1-2013
Abstract
OBJECTIVES: Abdominal aortic aneurysm (AAA), a dilatation of the infrarenal aorta, typically affects males >65 years. The pathobiological mechanisms of human AAA are poorly understood. The goal of this study was to identify novel pathways involved in the development of AAAs.
METHODS: A custom-designed 'AAA-chip' was used to assay 43 of the differentially expressed genes identified in a previously published microarray study between AAA (n = 15) and control (n = 15) infrarenal abdominal aorta. Protein analyses were performed on selected genes.
RESULTS: Altogether 38 of the 43 genes on the 'AAA-chip' showed significantly different expression. Novel validated genes in AAA pathobiology included ADCY7, ARL4C, BLNK, FOSB, GATM, LYZ, MFGE8, PRUNE2, PTPRC, SMTN, TMODI and TPM2. These genes represent a wide range of biological functions, such as calcium signaling, development and differentiation, as well as cell adhesion not previously implicated in AAA pathobiology. Protein analyses for GATM, CD4, CXCR4, BLNK, PLEK, LYZ, FOSB, DUSP6, ITGA5 and PTPRC confirmed the mRNA findings.
CONCLUSION: The results provide new directions for future research into AAA pathogenesis to study the role of novel genes confirmed here. New treatments and diagnostic tools for AAA could potentially be identified by studying these novel pathways.
Volume
80
Issue
1
First Page
1
Last Page
10
ISSN
1423-0291
Published In/Presented At
Hinterseher, I., Erdman, R., Elmore, J. R., Stahl, E., Pahl, M. C., Derr, K., Golden, A., Lillvis, J. H., Cindric, M. C., Jackson, K., Bowen, W. D., Schworer, C. M., Chernousov, M. A., Franklin, D. P., Gray, J. L., Garvin, R. P., Gatalica, Z., Carey, D. J., Tromp, G., & Kuivaniemi, H. (2013). Novel pathways in the pathobiology of human abdominal aortic aneurysms. Pathobiology : journal of immunopathology, molecular and cellular biology, 80(1), 1–10. https://doi.org/10.1159/000339303
Disciplines
Medicine and Health Sciences
PubMedID
22797469
Department(s)
Department of Surgery
Document Type
Article