Preformed antibodies to alpha1,3-galactosyltransferase gene-knockout (GT-KO) pig cells in humans, baboons, and monkeys: implications for xenotransplantation.
Publication/Presentation Date
10-1-2005
Abstract
OBJECTIVE: The aim of our study was to determine the prevalence and cytotoxicity of primate antibodies directed to antigens other than Galalpha1,3Gal (Gal), termed nonGal antigens.
METHODS: Sera from human, baboon, and cynomolgus monkeys were tested by flow cytometry for IgM and IgG binding to both wild-type (WT) and GT-KO pig peripheral mononuclear cells (PBMC). Also, complement-dependent cytotoxicity assays were performed.
RESULTS: All species demonstrated significantly higher antibody binding and cytotoxicity to WT cells compared to GT-KO cells (P < .01). Cynomolgus monkeys had significantly higher IgM binding to WT and GT-KO cells than did baboons or humans (P < .01). Furthermore, approximately 50% of both human and baboon sera proved to be lytic to GT-KO cells, compared to 76% of monkey sera (P < .01).
CONCLUSIONS: We confirm the advantage of using GT-KO pig grafts over WT pig grafts. However, our results suggest that, compared to the cynomolgus monkey, the baboon may be a more suitable model to study antibody-mediated rejection of GT-KO pig grafts.
Volume
37
Issue
8
First Page
3514
Last Page
3515
ISSN
0041-1345
Published In/Presented At
Rood, P. P., Hara, H., Ezzelarab, M., Busch, J., Zhu, X., Ibrahim, Z., Ball, S., Ayares, D., Awwad, M., & Cooper, D. K. (2005). Preformed antibodies to alpha1,3-galactosyltransferase gene-knockout (GT-KO) pig cells in humans, baboons, and monkeys: implications for xenotransplantation. Transplantation proceedings, 37(8), 3514–3515. https://doi.org/10.1016/j.transproceed.2005.09.082
Disciplines
Medicine and Health Sciences
PubMedID
16298646
Department(s)
Department of Surgery
Document Type
Article