Cyclic AMP-mobilizing agents and glucocorticoids modulate human smooth muscle cell migration.

Authors

• Elena A Goncharova
• Charlotte K Billington
• Carla Irani
• Alexander V Vorotnikov
• Vsevolod A Tkachuk
• Raymond B Penn
• Vera P Krymskaya
• Reynold A Panettieri

Publication/Presentation Date

7-1-2003

Abstract

Hyperplasia and cell migration of smooth muscle are features of both airway and pulmonary vascular diseases. The precise cellular and molecular mechanisms that regulate smooth muscle migration in the lungs remain unknown. In this study, we examined the effect of cAMP-mobilizing agents and steroids on smooth muscle cell migration. Platelet-derived growth factor (PDGF), transforming growth factor-alpha, vascular endothelial growth factor, and basic fibroblast growth factor significantly stimulated cell migration in pulmonary vascular smooth muscle (PVSM) cells. Airway smooth muscle (ASM) migration was also stimulated by PDGF, transforming growth factor-alpha, and basic fibroblast growth factor, but vascular endothelial growth factor was without effect. Interestingly, the smooth muscle mitogen thrombin did not stimulate migration of either cell type. Agents capable of elevating intracellular cAMP inhibited basal (unstimulated) cell migration in both cell types, whereas their effects on PDGF-stimulated migration were more variable. Prostaglandin E2, salmeterol, and the phosphodiesterase type 4 inhibitor cilomolast inhibited basal ASM and PVSM migration by 30-60%. Prostaglandin E2 and cilomolast also inhibited PDGF-stimulated migration of ASM and PVSM cells, but salmeterol was without effect. Preincubation of ASM cells with dexamethasone or fluticasone inhibited basal and PDGF-stimulated migration, and enabled an inhibitory effect of salmeterol on PDGF-induced cell migration. Steroids alone did not stimulate cAMP production or cAMP/PKA-dependent gene transcription (CRE-Luc activity), but slightly augmented salmeterol-stimulated CRE-Luc activity. Collectively, these findings demonstrate that cAMP-mobilizing agents and steroids modulate human smooth muscle cell migration, likely by distinct mechanisms.

Volume

29

Issue

1

First Page

19

Last Page

27

ISSN

1044-1549

Published In/Presented At

Goncharova, E. A., Billington, C. K., Irani, C., Vorotnikov, A. V., Tkachuk, V. A., Penn, R. B., Krymskaya, V. P., & Panettieri, R. A., Jr (2003). Cyclic AMP-mobilizing agents and glucocorticoids modulate human smooth muscle cell migration. American journal of respiratory cell and molecular biology, 29(1), 19–27. https://doi.org/10.1165/rcmb.2002-0254OC

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

12600820

Department(s)

Administration and Leadership

Document Type

Article