Isolation of an allele of reeler by insertional mutagenesis.
Publication/Presentation Date
11-8-1994
Abstract
Reeler (rl) is an autosomal recessive mutation that affects migration of postmitotic neurons in the mouse central nervous system. The reeler (rl/rl) mouse displays a disruption of laminar structures in both the cerebellum and the forebrain and it exhibits tremors, dystonia, and ataxia. The molecular basis of the reeler phenotype is unknown because the gene involved has not yet been identified. We report here the isolation and characterization of an allele of rl, reelertransgene (rltg). This allele was generated by the fortuitous insertion of a transgene, supfos (sf), into the mouse rl locus. Crosses between rl/+ and rltg/+ mice yielded offspring that exhibited the reeler phenotype, indicating that rl and rltg are allelic. We cloned the genomic sequences flanking the transgene insertion site from the rltg/rltg mouse genome. Chromosomal mapping studies revealed that the 5' flanking cellular sequence maps to a locus, D5Gmr1, that lies in a region of mouse chromosome 5 that also contains the rl locus. Southern blot analysis using a probe derived from the D5Gmr1 locus revealed no gross structural rearrangement in the rl locus. Thus, unlike the two rl alleles described previously, rltg provides a molecular probe that can now be used to identify and isolate the rl gene.
Volume
91
Issue
23
First Page
11050
Last Page
11054
ISSN
0027-8424
Published In/Presented At
Miao, G. G., Smeyne, R. J., D'Arcangelo, G., Copeland, N. G., Jenkins, N. A., Morgan, J. I., & Curran, T. (1994). Isolation of an allele of reeler by insertional mutagenesis. Proceedings of the National Academy of Sciences of the United States of America, 91(23), 11050–11054. https://doi.org/10.1073/pnas.91.23.11050
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
7972007
Department(s)
Administration and Leadership
Document Type
Article