A monoclonal antibody to Schwann cell surface membrane recognizes a cAMP inducible epitope.
Publication/Presentation Date
11-10-1987
Abstract
A monoclonal antibody to the surface membrane of rat Schwann cells was generated. This antibody bound to Schwann cell surface but not to fibroblasts in mixed neonatal sciatic nerve cultures. It did not bind to cells in newborn rat brain or corpus callosum cultures that contained oligodendrocytes, astrocytes, fibroblasts and neurons. The target antigen for this monoclonal antibody disappeared from Schwann cell surface after 5 days in culture. At this time, addition of 10(-3) M 8-bromo cyclic AMP or 10(-3) M dibutyryl cyclic AMP resulted in the reappearance within 2 days, of the target antigen on Schwann cell surface membrane. The reappearance of this epitope parallels that of surface galactocerebroside on Schwann cells when incubated with cAMP analogues. Re-expression of the target antigen for this monoclonal antibody in the presence of cAMP is evidence that cAMP may be a signal for synthesis of a variety of myelin molecules and as such could be a signal for myelination of axons by Schwann cells.
Volume
425
Issue
2
First Page
205
Last Page
211
ISSN
0006-8993
Published In/Presented At
Rostami, A., Sobue, G., Lisak, R. P., & Pleasure, D. E. (1987). A monoclonal antibody to Schwann cell surface membrane recognizes a cAMP inducible epitope. Brain research, 425(2), 205–211. https://doi.org/10.1016/0006-8993(87)90502-6
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
2448004
Department(s)
Administration and Leadership
Document Type
Article