The expression of cytokine mRNA in the cauda equina of Lewis rats with experimental allergic neuritis.
Publication/Presentation Date
4-15-1998
Abstract
Autoreactive CD4+ T cells can transfer experimental allergic neuritis (EAN) to naive recipients. In order to further analyze the role of these T cells and their corresponding cytokines in EAN, we studied the expression of mRNA for IFN-gamma, IL-4, and IL-10 in the cauda equina of rats with EAN using a quantitative competitive reverse transcriptase PCR method. Nerves were studied on days 0 (pre-immunization), 10 (disease onset), 13 (clinical progression), 16 (disease peak), as well as 20, 24, and 34 post immunization (recovery). IFN-gamma messages increased at disease onset and peaked at day 13 p.i. IL-10 message remained at a very low level at disease onset and surged at day 16. Both messages were low in recovery stage. IL-4 message was undetectable at any time point. These data suggest a pro-inflammatory role of IFN-gamma and anti-inflammatory role of IL-10 in EAN lesions. It is also possible that a clonal switch from Th1 to Th2 occurs in EAN lesions during the disease course.
Volume
84
Issue
2
First Page
223
Last Page
229
ISSN
0165-5728
Published In/Presented At
Fujioka, T., Jimi, T., Hilliard, B. A., Ventura, E. S., & Rostami, A. (1998). The expression of cytokine mRNA in the cauda equina of Lewis rats with experimental allergic neuritis. Journal of neuroimmunology, 84(2), 223–229. https://doi.org/10.1016/s0165-5728(97)00252-x
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
9628467
Department(s)
Administration and Leadership
Document Type
Article