Effect of multiple doses of rifampin on the [14C N-methyl] erythromycin breath test in healthy male volunteers.
Publication/Presentation Date
6-1-1998
Abstract
The erythromycin breath test (EBT), which measures 14CO2 produced from [14C N-methyl] erythromycin, is one of the most frequently employed measures to examine drug interactions involving cytochrome P450 3A4 (CYP3A). However, the reproducibility and reliability of this test, and the effects of drugs that alter CYP3A activity, continue to be defined. In this study, the reproducibility of the EBT was evaluated in eight healthy volunteers before and after oral administration of 600 mg of rifampin daily for 8 days. Two sequential EBT determinations performed 5 days apart before rifampin administration were highly reproducible. Rifampin induced CYP3A, reflected in a mean percent (+/- standard deviation) increase in EBT values of 86 +/- 30%. Recovery of enzyme function after discontinuation of rifampin for 17 days was manifested as a return of EBT values to preinduction levels. These results support the utility of EBT as a valid, reproducible, and reliable measure of CYP3A activity in vivo.
Volume
38
Issue
6
First Page
492
Last Page
495
ISSN
0091-2700
Published In/Presented At
Gharaibeh, M. N., Gillen, L. P., Osborne, B., Schwartz, J. I., & Waldman, S. A. (1998). Effect of multiple doses of rifampin on the [14C N-methyl] erythromycin breath test in healthy male volunteers. Journal of clinical pharmacology, 38(6), 492–495. https://doi.org/10.1002/j.1552-4604.1998.tb05785.x
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
9650537
Department(s)
Administration and Leadership
Document Type
Article