Tumor-Derived Extracellular Vesicles Require β1 Integrins to Promote Anchorage-Independent Growth.

Authors

• Rachel M DeRita
• Aejaz Sayeed
• Vaughn Garcia
• Shiv Ram Krishn
• Christopher D Shields
• Srawasti Sarker
• Andrea Friedman
• Peter McCue
• Sudheer Kumar Molugu
• Ulrich Rodeck
• Adam P Dicker
• Lucia R Languino

Publication/Presentation Date

4-26-2019

Abstract

The β1 integrins, known to promote cancer progression, are abundant in extracellular vesicles (EVs). We investigated whether prostate cancer (PrCa) EVs affect anchorage-independent growth and whether β1 integrins are required for this effect. Specifically using a cell-line-based genetic rescue and an in vivo PrCa model, we show that gradient-purified small EVs (sEVs) from either cancer cells or blood from tumor-bearing TRAMP (transgenic adenocarcinoma of the mouse prostate) mice promote anchorage-independent growth of PrCa cells. In contrast, sEVs from cultured PrCa cells harboring a short hairpin RNA to β1, from wild-type mice or from TRAMP mice carrying a β1 conditional ablation in the prostatic epithelium (β1

Volume

14

First Page

199

Last Page

209

ISSN

2589-0042

Published In/Presented At

DeRita, R. M., Sayeed, A., Garcia, V., Krishn, S. R., Shields, C. D., Sarker, S., Friedman, A., McCue, P., Molugu, S. K., Rodeck, U., Dicker, A. P., & Languino, L. R. (2019). Tumor-Derived Extracellular Vesicles Require β1 Integrins to Promote Anchorage-Independent Growth. iScience, 14, 199–209. https://doi.org/10.1016/j.isci.2019.03.022

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

30981115

Department(s)

Administration and Leadership

Document Type

Article