Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation.

Authors

• Srimeenakshi Srinivasan
• Ashish Yeri
• Pike See Cheah
• Allen Chung
• Kirsty Danielson
• Peter De Hoff
• Justyna Filant
• Clara D Laurent
• Lucie D Laurent
• Rogan Magee
• Courtney Moeller
• Venkatesh L Murthy
• Parham Nejad
• Anu Paul
• Isidore Rigoutsos
• Rodosthenis Rodosthenous
• Ravi V Shah
• Bridget Simonson
• Cuong To
• David Wong
• Irene K Yan
• Xuan Zhang
• Leonora Balaj
• Xandra O Breakefield
• George Daaboul
• Roopali Gandhi
• Jodi Lapidus
• Eric Londin
• Tushar Patel
• Robert L Raffai
• Anil K Sood
• Roger P Alexander
• Saumya Das
• Louise C Laurent

Publication/Presentation Date

4-4-2019

Abstract

Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of 10 exRNA isolation methods across 5 biofluids revealed marked differences in the complexity and reproducibility of the resulting small RNA-seq profiles. The relative efficiency with which each method accessed different exRNA carrier subclasses was determined by estimating the proportions of extracellular vesicle (EV)-, ribonucleoprotein (RNP)-, and high-density lipoprotein (HDL)-specific miRNA signatures in each profile. An interactive web-based application (miRDaR) was developed to help investigators select the optimal exRNA isolation method for their studies. miRDar provides comparative statistics for all expressed miRNAs or a selected subset of miRNAs in the desired biofluid for each exRNA isolation method and returns a ranked list of exRNA isolation methods prioritized by complexity, expression level, and reproducibility. These results will improve reproducibility and stimulate further progress in exRNA biomarker development.

Volume

177

Issue

2

First Page

446

Last Page

462

ISSN

1097-4172

Published In/Presented At

Srinivasan, S., Yeri, A., Cheah, P. S., Chung, A., Danielson, K., De Hoff, P., Filant, J., Laurent, C. D., Laurent, L. D., Magee, R., Moeller, C., Murthy, V. L., Nejad, P., Paul, A., Rigoutsos, I., Rodosthenous, R., Shah, R. V., Simonson, B., To, C., Wong, D., … Laurent, L. C. (2019). Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell, 177(2), 446–462.e16. https://doi.org/10.1016/j.cell.2019.03.024

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

30951671

Department(s)

Administration and Leadership

Document Type

Article