Intravascular immune cell labeling as a critical methodological tool for characterizing CD4
Publication/Presentation Date
1-1-2026
Abstract
INTRODUCTION: Growing evidence highlights the lung -brain axis as a key contributor to CNS autoimmunity. However, accurate characterization of lung immune cells remains technically challenging because standard transcardial perfusion does not eliminate the lung's extensive marginated intravascular leukocyte pool, allowing circulating cells to contaminate analyses of the lung parenchyma.
METHODS: Experimental autoimmune encephalomyelitis (EAE) was induced in mice by immunization with MOG
RESULTS: We performed
DISCUSSION: These findings demonstrate that transcardial perfusion alone is insufficient to accurately distinguish lung parenchymal from intravascular immune cells. We propose that
Volume
17
First Page
1950269
Last Page
1950269
ISSN
1664-3224
Published In/Presented At
Azizi, G., & Rostami, A. (2026). Intravascular immune cell labeling as a critical methodological tool for characterizing CD4+ T cells in the lung in EAE. Frontiers in immunology, 17, 1950269. https://doi.org/10.3389/fimmu.2026.1950269
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
42780564
Department(s)
Administration and Leadership
Document Type
Article