Defining Transcriptomic Heterogeneity between Left and Right Ventricle-Derived Cardiac Fibroblasts.
Publication/Presentation Date
2-10-2024
Abstract
Cardiac fibrosis is a key aspect of heart failure, leading to reduced ventricular compliance and impaired electrical conduction in the myocardium. Various pathophysiologic conditions can lead to fibrosis in the left ventricle (LV) and/or right ventricle (RV). Despite growing evidence to support the transcriptomic heterogeneity of cardiac fibroblasts (CFs) in healthy and diseased states, there have been no direct comparisons of CFs in the LV and RV. Given the distinct natures of the ventricles, we hypothesized that LV- and RV-derived CFs would display baseline transcriptomic differences that influence their proliferation and differentiation following injury. Bulk RNA sequencing of CFs isolated from healthy murine left and right ventricles indicated that LV-derived CFs may be further along the myofibroblast transdifferentiation trajectory than cells isolated from the RV. Single-cell RNA-sequencing analysis of the two populations confirmed that
Volume
13
Issue
4
ISSN
2073-4409
Published In/Presented At
Dewar, M. B., Ehsan, F., Izumi, A., Zhang, H., Zhou, Y. Q., Shah, H., Langburt, D., Suresh, H., Wang, T., Hacker, A., Hinz, B., Gillis, J., Husain, M., & Heximer, S. P. (2024). Defining Transcriptomic Heterogeneity between Left and Right Ventricle-Derived Cardiac Fibroblasts. Cells, 13(4), 327. https://doi.org/10.3390/cells13040327
Disciplines
Medicine and Health Sciences
PubMedID
38391940
Department(s)
Fellows and Residents
Document Type
Article
