Cyclic Di-AMP Affects Cell Membrane Integrity of Streptococcus pneumoniae.
Publication/Presentation Date
6-27-2025
Abstract
Competence is an important bioprocess for Streptococcus pneumoniae. Previously, we demonstrated that the bacterial second messenger cyclic di-adenosine monophosphate (c-di-AMP) modulates pneumococcal competence. Surprisingly, cdaA*, a strain producing less c-di-AMP due to a point mutation in the diadenylate cyclase CdaA, is susceptible to competence-stimulating peptide (CSP). In this study, we screened cdaA* suppressor mutants resistant to CSP to explore the underlying mechanism. Of 14 clones sequenced, nine clones possessed mutations in the c-di-AMP phosphodiesterase Pde1, indicating that the susceptibility to CSP of the cdaA* strain is correlated to c-di-AMP levels. Another two clones exhibited a mutation in FabT, a transcription factor controlling cell membrane fatty acid biosynthesis. We further showed that deletion of fabT, disruption of the FabT-binding site within the P
ISSN
1365-2958
Published In/Presented At
Zarrella, T. M., Gao, J., Forrest, N., Crosbourne, E., Cui, K., & Bai, G. (2025). Cyclic Di-AMP Affects Cell Membrane Integrity of Streptococcus pneumoniae. Molecular microbiology, 10.1111/mmi.70003. Advance online publication. https://doi.org/10.1111/mmi.70003
Disciplines
Medicine and Health Sciences
PubMedID
40576446
Department(s)
Fellows and Residents
Document Type
Article