Total synthesis of a functional designer eukaryotic chromosome.
Publication/Presentation Date
4-4-2014
Abstract
Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology.
Volume
344
Issue
6179
First Page
55
Last Page
58
ISSN
1095-9203
Published In/Presented At
Annaluru, N., Muller, H., Mitchell, L. A., Ramalingam, S., Stracquadanio, G., Richardson, S. M., Dymond, J. S., Kuang, Z., Scheifele, L. Z., Cooper, E. M., Cai, Y., Zeller, K., Agmon, N., Han, J. S., Hadjithomas, M., Tullman, J., Caravelli, K., Cirelli, K., Guo, Z., London, V., … Chandrasegaran, S. (2014). Total synthesis of a functional designer eukaryotic chromosome. Science (New York, N.Y.), 344(6179), 55–58. https://doi.org/10.1126/science.1249252
Disciplines
Medicine and Health Sciences
PubMedID
24674868
Department(s)
Fellows and Residents
Document Type
Article