High-Throughput Immunoassays for Cavin-4 IgG: A Diagnostic Tool for Immune-Mediated Rippling Muscle Disease.
Publication/Presentation Date
4-1-2025
Abstract
Cavin-4 was identified as a potential autoantigen for immune-mediated rippling muscle disease (iRMD). To validate this, we developed and tested various immunoassays, including a cell-based assay (CBA), cavin-4 recombinant protein ELISA, and multi-peptide ELISA. Among 19 iRMD patients, all exhibited muscle rippling, and 13 had percussion-induced mounding. All immunoassays demonstrated clinical and analytical specificities greater than 95%. The protein ELISA had the highest sensitivity (94.7%) and specificity (99.9%), outperforming CBA (sensitivity 89.5%, specificity 99.6%) and the multi-peptide ELISA (sensitivity 79.0%, specificity 97.2%). Our results suggest that the cavin-4 protein ELISA is a promising tool for high-throughput clinical testing in iRMD.
Volume
12
Issue
4
First Page
876
Last Page
880
ISSN
2328-9503
Published In/Presented At
LaFrance-Corey, R. G., Kherbek, H., Harinesan, N., Milone, M., Paramasivan, N. K., Sarker, P., Knight, A. M., Karsten, C., Dasari, S., Liewluck, T., Litchy, W. J., Pittock, S. J., Mills, J. R., & Dubey, D. (2025). High-Throughput Immunoassays for Cavin-4 IgG: A Diagnostic Tool for Immune-Mediated Rippling Muscle Disease. Annals of clinical and translational neurology, 12(4), 876–880. https://doi.org/10.1002/acn3.70012
Disciplines
Medicine and Health Sciences
PubMedID
39957412
Department(s)
Fellows and Residents
Document Type
Article