IL-13Ralpha2 is a glioma-restricted receptor for interleukin-13.
Publication/Presentation Date
1-1-2002
Abstract
We have found that binding sites for interleukin-13 (IL-13) are overexpressed in a vast majority of high-grade astrocytomas (HGAs). These binding sites for IL-13 are distinct from the physiological receptor in that it does not bind IL-4. We also demonstrated that IL-13 receptor alpha 2 protein chain (IL-13Ralpha2), an IL-4-independent receptor for IL-13, is abundant among HGAs, but not in normal organs. To examine if IL-13Ralpha2 is the tumor-associated site for IL-13, we stably transfected normal Chinese hamster ovary (CHO) cells and glioma G-26 cells to express either human (h) or murine (m) IL-13Ralpha2. CHO-hIL-13Ralpha2(+) cells and G-26-h/mIL-13Ralpha2(+) cells, and not CHO and G-26 parental or mock-transfected cells, specifically bound IL-13 in an IL-4-independent manner. The IL-13Ralpha2(+) cells also became highly susceptible to the killing by an IL-13-based cytotoxic fusion protein. In loss of function studies, a HGA cell line, SNB-19, was transfected with antisense (as) hIL-13Ralpha2. as-SNB-19-hIL-13Ralpha2(+) cells lost their natural affinity towards IL-13 and became resistant to IL-13-based cytotoxins. The fact, that IL-13Ralpha2-positive cells bind IL-13 independent of IL-4, become susceptible to IL-13 cytotoxins, and cells deprived of IL-13Ralpha2 receptor lose these features, demonstrates that IL-13Ralpha2 is the brain tumor-associated receptor for IL-13.
Volume
4
Issue
5
First Page
388
Last Page
399
ISSN
1522-8002
Published In/Presented At
Mintz, A., Gibo, D. M., Slagle-Webb, B., Christensen, N. D., & Debinski, W. (2002). IL-13Ralpha2 is a glioma-restricted receptor for interleukin-13. Neoplasia (New York, N.Y.), 4(5), 388–399. https://doi.org/10.1038/sj.neo.7900234
Disciplines
Medicine and Health Sciences
PubMedID
12192597
Department(s)
Fellows and Residents
Document Type
Article