Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
Publication/Presentation Date
8-15-2016
Abstract
Rho GAPs are important regulators of Rho GTPases, which are involved in various steps of cytokinesis and other processes. However, regulation of Rho-GAP cellular localization and function is not fully understood. Here we report the characterization of a novel coiled-coil protein Rng10 and its relationship with the Rho-GAP Rga7 in fission yeast. Both rng10Δ and rga7Δ result in defective septum and cell lysis during cytokinesis. Rng10 and Rga7 colocalize on the plasma membrane at the cell tips during interphase and at the division site during cell division. Rng10 physically interacts with Rga7 in affinity purification and coimmunoprecipitation. Of interest, Rga7 localization is nearly abolished without Rng10. Moreover, Rng10 and Rga7 work together to regulate the accumulation and dynamics of glucan synthases for successful septum formation in cytokinesis. Our results show that cellular localization and function of the Rho-GAP Rga7 are regulated by a novel protein, Rng10, during cytokinesis in fission yeast.
Volume
27
Issue
16
First Page
2528
Last Page
2541
ISSN
1939-4586
Published In/Presented At
Liu, Y., Lee, I. J., Sun, M., Lower, C. A., Runge, K. W., Ma, J., & Wu, J. Q. (2016). Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis. Molecular biology of the cell, 27(16), 2528–2541. https://doi.org/10.1091/mbc.E16-03-0156
Disciplines
Medicine and Health Sciences
PubMedID
27385337
Department(s)
Fellows and Residents
Document Type
Article