Sequence homology between HLA-bound cytomegalovirus and human peptides: A potential trigger for alloreactivity.
Human cytomegalovirus (hCMV) reactivation may often coincide with the development of graft-versus-host-disease (GVHD) in stem cell transplantation (SCT). Seventy seven SCT donor-recipient pairs (DRP) (HLA matched unrelated donor (MUD), n = 50; matched related donor (MRD), n = 27) underwent whole exome sequencing to identify single nucleotide polymorphisms (SNPs) generating alloreactive peptide libraries for each DRP (9-mer peptide-HLA complexes); Human CMV CROSS (Cross-Reactive Open Source Sequence) database was compiled from NCBI; HLA class I binding affinity for each DRPs HLA was calculated by NetMHCpan 2.8 and hCMV- derived 9-mers algorithmically compared to the alloreactive peptide-HLA complex libraries. Short consecutive (≥6) amino acid (AA) sequence homology matching hCMV to recipient peptides was considered for HLA-bound-peptide (IC50
Published In/Presented At
Hall, C. E., Koparde, V. N., Jameson-Lee, M., Elnasseh, A. G., Scalora, A. F., Kobulnicky, D. J., Serrano, M. G., Roberts, C. H., Buck, G. A., Neale, M. C., Nixon, D. E., & Toor, A. A. (2017). Sequence homology between HLA-bound cytomegalovirus and human peptides: A potential trigger for alloreactivity. PloS one, 12(8), e0178763. https://doi.org/10.1371/journal.pone.0178763
Medicine and Health Sciences
Department of Medicine, Hematology-Medical Oncology Division