Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer.

Authors

• Katia Cheaito
• Hisham F Bahmad
• Ola Hadadeh
• Hiba Msheik
• Alissar Monzer
• Farah Ballout
• Christelle Dagher
• Talar Telvizian MD, Lehigh Valley Health NetworkFollow
• Nour Saheb
• Ayman Tawil
• Marwan El-Sabban
• Albert El-Hajj
• Deborah Mukherji
• Mohamed Al-Sayegh
• Wassim Abou-Kheir

Publication/Presentation Date

1-1-2022

Abstract

Three-dimensional (3D) organoid culture systems are emerging as potential reliable tools to investigate basic developmental processes of human disease, especially cancer. The present study used established and modified culture conditions to report successful generation and characterization of patient-derived organoids from fresh primary tissue specimens of patients with treatment-naïve prostate cancer (PCa). Fresh tissue specimens were collected, digested enzymatically and the resulting cell suspensions were plated in a 3D environment using Matrigel as an extracellular matrix. Previously established 12-factor medium for organoid culturing was modified to create a minimal 5-factor medium. Organoids and corresponding tissue specimens were characterized using transcriptomic analysis, immunofluorescent analysis, and immunohistochemistry. Furthermore, patient-derived organoids were used to assess the drug response. Treatment-naïve patient-derived PCa organoids were obtained from fresh radical prostatectomy specimens. These PCa organoids mimicked the heterogeneity of corresponding parental tumor tissue. Histopathological analysis demonstrated similar tissue architecture and cellular morphology, as well as consistent immunohistochemical marker expression. Also, the results confirmed the potential of organoids as an

Volume

23

Issue

1

First Page

6

Last Page

6

ISSN

1792-1082

Published In/Presented At

Cheaito, K., Bahmad, H. F., Hadadeh, O., Msheik, H., Monzer, A., Ballout, F., Dagher, C., Telvizian, T., Saheb, N., Tawil, A., El-Sabban, M., El-Hajj, A., Mukherji, D., Al-Sayegh, M., & Abou-Kheir, W. (2022). Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer. Oncology letters, 23(1), 6. https://doi.org/10.3892/ol.2021.13124

Disciplines

Medicine and Health Sciences

PubMedID

34820005

Department(s)

Department of Medicine, Hematology-Medical Oncology Division

Document Type

Article