Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts.
Publication/Presentation Date
7-30-1998
Abstract
Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g., interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-2 by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-2 mRNA in human gingival fibroblasts.
Volume
248
Issue
3
First Page
450
Last Page
453
ISSN
0006-291X
Published In/Presented At
Fowler, M. J., Jr, Neff, M. S., Borghaei, R. C., Pease, E. A., Mochan, E., & Thornton, R. D. (1998). Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts. Biochemical and biophysical research communications, 248(3), 450–453. https://doi.org/10.1006/bbrc.1998.8988
Disciplines
Medicine and Health Sciences
PubMedID
9703944
Department(s)
Department of Medicine
Document Type
Article