Characterization of a chicken cDNA encoding the retinoblastoma gene product.
Publication/Presentation Date
5-17-1994
Abstract
We have isolated a chicken cDNA that encodes the retinoblastoma susceptibility gene product (RB). The predicted amino acid sequence of the chicken RB protein is highly similar to that of the mouse, human and Xenopus RB proteins in regions of known functions; however, chicken RB has distinct species-specific differences, including a shorter N-terminal region as compared to the mouse and human RB proteins. In vitro-translated chicken RB co-migrates on SDS-polyacrylamide gels with endogenous RB synthesized in transformed chicken spleen cells. Finally, chicken RB is located in the nucleus of chicken embryo fibroblasts when overexpressed from a retroviral vector.
Volume
1218
Issue
1
First Page
82
Last Page
86
ISSN
0006-3002
Published In/Presented At
Feinstein R, Bolton WK, Quinones JN, Mosialos G, Sif S, Huff JL, Capobianco AJ, Gilmore TD. Characterization of a chicken cDNA encoding the retinoblastoma gene product. Biochim Biophys Acta. 1994 May 17;1218(1):82-6. doi: 10.1016/0167-4781(94)90103-1. PMID: 8193168.
Disciplines
Medicine and Health Sciences
PubMedID
8193168
Department(s)
Department of Obstetrics and Gynecology
Document Type
Article