Characterization of a chicken cDNA encoding the retinoblastoma gene product.

Publication/Presentation Date

5-17-1994

Abstract

We have isolated a chicken cDNA that encodes the retinoblastoma susceptibility gene product (RB). The predicted amino acid sequence of the chicken RB protein is highly similar to that of the mouse, human and Xenopus RB proteins in regions of known functions; however, chicken RB has distinct species-specific differences, including a shorter N-terminal region as compared to the mouse and human RB proteins. In vitro-translated chicken RB co-migrates on SDS-polyacrylamide gels with endogenous RB synthesized in transformed chicken spleen cells. Finally, chicken RB is located in the nucleus of chicken embryo fibroblasts when overexpressed from a retroviral vector.

Volume

1218

Issue

1

First Page

82

Last Page

86

ISSN

0006-3002

Disciplines

Medicine and Health Sciences

PubMedID

8193168

Department(s)

Department of Obstetrics and Gynecology

Document Type

Article

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