Determination of zinc in whole blood, plasma and serum using Zeeman effect flame atomic absorption spectroscopy.
Publication/Presentation Date
10-15-1985
Abstract
Methods are presented for the determination of zinc in whole blood, plasma and serum using Zeeman effect flame atomic absorption spectroscopy and a flame microsampling funnel. Whole blood was diluted 1/25 with 0.10 mol/l hydrochloric acid; plasma and serum were diluted 1/5 with deionized water. Concentrations could be read directly from standards prepared in human blood pools. The within-run relative standard deviation (RSD) was 0.50%, 0.82% and 0.61% for whole blood specimens with concentrations of 4 360 micrograms/l, 5 967 micrograms/l and 8 297 micrograms/l, respectively. The within-run RSD was 2.09%, 1.16% and 0.62% for plasma specimens with zinc concentrations of 442 micrograms/l, 976 micrograms/l and 1 731 micrograms/l, respectively. The within-run RSD was 1.18%, 1.22% and 1.02% for serum specimens with zinc concentrations of 492 micrograms/l, 1 023 micrograms/l and 1 533 micrograms/l, respectively. The detection limit was 3.6 micrograms/l.
Volume
151
Issue
3
First Page
237
Last Page
243
ISSN
0009-8981
Published In/Presented At
Liska, S. K., Kerkay, J., & Pearson, K. H. (1985). Determination of zinc in whole blood, plasma and serum using Zeeman effect flame atomic absorption spectroscopy. Clinica chimica acta; international journal of clinical chemistry, 151(3), 237–243. https://doi.org/10.1016/0009-8981(85)90086-5
Disciplines
Medicine and Health Sciences
PubMedID
4053384
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article