Determination of zinc and copper in urine using Zeeman effect flame atomic absorption spectroscopy.
Publication/Presentation Date
10-15-1985
Abstract
Zinc and copper were determined in urine using polarized Zeeman effect flame atomic absorption spectrophotometry. For the zinc assay, urine was diluted 1/10 with deionized water. Concentrations could be determined by comparison to standards in a salt matrix or in a commercial urine control. The linearity of the assay was 350 micrograms/l, the detection limit was 1.2 micrograms/l and the within-run relative standard deviation (RSD) was 2.08%, 3.06%, 0.71% and 1.29% for specimens with zinc concentrations of 202 micrograms/l, 206 micrograms/l, 1 003 micrograms/l and 1 032 micrograms/l, respectively. The between-run RSD was 2.34% for a mean zinc concentration of 461 micrograms/l. For the copper assay, urine was aspirated directly and concentrations were determined by standard additions. The linearity of the assay was 5 000 micrograms/l, the detection limit was 4.6 micrograms/l and the within-run RSD was 24.49%, 16.10%, 4.00% and 3.19% for specimens with copper concentrations of 9.8 micrograms/l, 11.8 micrograms/l, 50.0 micrograms/l and 50.2 micrograms/l, respectively. The between-run RSD was 8.78% and 4.72% for specimens with copper concentrations of 21.1 micrograms/l and 40.3 micrograms/l, respectively.
Volume
151
Issue
3
First Page
231
Last Page
236
ISSN
0009-8981
Published In/Presented At
Liska, S. K., Kerkay, J., & Pearson, K. H. (1985). Determination of zinc and copper in urine using Zeeman effect flame atomic absorption spectroscopy. Clinica chimica acta; international journal of clinical chemistry, 151(3), 231–236. https://doi.org/10.1016/0009-8981(85)90085-3
Disciplines
Medicine and Health Sciences
PubMedID
4053383
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article