Cloning and identification of bacteriophage T4 gene 2 product gp2 and action of gp2 on infecting DNA in vivo.
Publication/Presentation Date
1-1-1989
Abstract
We sequenced bacteriophage T4 genes 2 and 3 and the putative C-terminal portion of gene 50. They were found to have appropriate open reading frames directed counterclockwise on the T4 map. Mutations in genes 2 and 64 were shown to be in the same open reading frame, which we now call gene 2. This gene codes for a protein of 27,068 daltons. The open reading frame corresponding to gene 3 codes for a protein of 20,634 daltons. Appropriate bands on polyacrylamide gels were identified at 30 and 20 kilodaltons, respectively. We found that the product of the cloned gene 2 can protect T4 DNA double-stranded ends from exonuclease V action.
Volume
171
Issue
1
First Page
488
Last Page
497
ISSN
0021-9193
Published In/Presented At
Lipinska, B., Rao, A. S., Bolten, B. M., Balakrishnan, R., & Goldberg, E. B. (1989). Cloning and identification of bacteriophage T4 gene 2 product gp2 and action of gp2 on infecting DNA in vivo. Journal of bacteriology, 171(1), 488–497. https://doi.org/10.1128/jb.171.1.488-497.1989
Disciplines
Medicine and Health Sciences
PubMedID
2644202
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article