Filter paper-based insulin-like growth factor assay.
Publication/Presentation Date
8-1-2015
Abstract
This study assessed validity, reliability, and feasibility of a filter paper blood spot insulin-like growth factor 1 (IGF-1) assay for retinopathy of prematurity (ROP) research. Blood samples were collected from 45 healthy children. Half of each sample was spun to obtain serum; half was applied to filter cards and stored for varying times and at different temperatures. IGF-1 assays were performed using a commercially available kit. Intraclass correlation between blood spot and serum IGF-1 values was high (0.97) for validity, and the mean differences were low for test-retest reliability. Time (up to 25 days) and temperature (4° C to 37° C) had no significant effect on sample stability. Feasibility was further assessed in a second cohort study of 74 premature infants being screened for ROP. A total of 817 filter card samples were successfully collected and transported to a central lab, where IGF-1 assays were successfully performed.
Volume
19
Issue
4
First Page
363
Last Page
365
ISSN
1528-3933
Published In/Presented At
Jensen, A. K., Coleman, C., Stokes, D., Ying, G. S., Huang, J., Kuhn, I., & Binenbaum, G. (2015). Filter paper-based insulin-like growth factor assay. Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus, 19(4), 363–365. https://doi.org/10.1016/j.jaapos.2015.03.022
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
26235792
Department(s)
Department of Pediatrics
Document Type
Article