USF-LVHN SELECT
Dose-in dependent threshold illumination for non-invasive time-lapse fluorescence imaging of live cells
Publication/Presentation Date
7-1-2021
Abstract
Fluorescent microscopy employs monochromatic light for excitation, which can adversely affect the cells being observed. We reported earlier that fibroblasts relax their contractile force in response to green light of typical intensity. Here we show that such effects are independent of extracellular matrix and cell lines. In addition, we establish a threshold intensity that elicits minimal or no adverse effect on cell contractility even for long-time exposure. This threshold intensity is wavelength dependent. We cultured fibroblasts on soft 2D elastic hydrogels embedded with fluorescent beads to trace substrate deformation and cell forces. The beads move towards cell center when cells contract, but they move away when cells relax. We use relaxation/contraction ratio (
Volume
46
ISSN
2352-4316
Published In/Presented At
Emon, M. A. B., Knoll, S., Doha, U., Ladehoff, L., Lalonde, L., Baietto, D., Sivaguru, M., Bhargava, R., & Saif, M. T. A. (2021). Dose-independent threshold illumination for non-invasive time-lapse fluorescence imaging of live cells. Extreme Mechanics Letters, 46, 101249. https://doi.org/10.1016/j.eml.2021.101249
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
34095408
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article