Proteomic Analysis of BMAC from the Iliac Crest and Humeral Head Using Support Vector Machines.

Publication/Presentation Date

8-12-2025

Abstract

The aim of this study was to analyze the proteomic differences in bone marrow aspirate concentrate (BMAC) between extraction sites, specifically, the iliac crest and humeral head. Using support vector machine (SVM) models, SVM-(linear) and SVM-(rbf), we identified six key proteins (MCSF, CD14, CD40, CD163, Ephrin-A4, and Matrilin-3) as essential for distinguishing between these sites. The results indicate that site-to-site proteomic differences are likely influenced by the surrounding tissues of each extraction site. Higher levels of chondrogenic and osteogenic proteins in the humeral head BMAC in addition to immune-modulatory proteins in the iliac crest BMAC suggest site-specific proteomic profiles. This study provides insights that may assist clinicians in selecting site-appropriate BMAC for therapeutic applications and inform future research into BMAC proteomics.

Volume

10

Issue

31

First Page

34975

Last Page

34985

ISSN

2470-1343

Disciplines

Medicine and Health Sciences

PubMedID

40821561

Department(s)

Department of Surgery

Document Type

Article

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