Sepsis-induced lung injury in rats increases alveolar epithelial vulnerability to stretch.

Publication/Presentation Date

6-1-2006

Abstract

OBJECTIVE: Previous in vitro models have shown that cellular deformation causes dose-dependent injury and death in healthy rat alveolar epithelial cells (AECs). We compared the viability of AECs from septic rats with those from nonseptic rats after 1 hr of cyclic equibiaxial stretch. We hypothesized that sepsis would increase stretch-induced cell death.

DESIGN: Laboratory investigation.

SETTING: University research laboratory.

SUBJECTS: Thirty-seven male Sprague-Dawley rats weighing 240-260 g.

INTERVENTIONS: Anesthetized rats were subjected to cecal ligation and double puncture (2CLP) or sham laparotomy without cecal ligation or puncture (sham). After 24 or 48 hrs, AECs were isolated, seeded in custom wells, and maintained in culture for 48 hrs before study. AECs were stretched cyclically (15/min) to a 0%, 12%, 25%, or 37% change in surface area (DeltaSA) for 1 hr. Cell viability, phenotypic markers, and nuclear factor-kappaB intracellular localization were assessed using fluorescent immunocytochemistry.

MEASUREMENTS AND MAIN RESULTS: Phase and fluorescent images were evaluated for all studies. Response to stretch was the same at 24 and 48 hrs after 2CLP. Relative to sham, 2CLP significantly increased cell death at 25 and 37% DeltaSA (p

CONCLUSIONS: AECs isolated from septic rats are more vulnerable to mechanical deformation injury than AECs from nonseptic animals.

Volume

34

Issue

6

First Page

1746

Last Page

1751

ISSN

0090-3493

Disciplines

Medicine and Health Sciences | Pediatrics

PubMedID

16625134

Department(s)

Department of Pediatrics

Document Type

Article

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