Induction of apoptosis of human brain microvascular endothelial cells by shiga toxin 1.
Publication/Presentation Date
1-1-2003
Abstract
Brain injury is the most frequent cause of mortality among patients with the hemolytic-uremic syndrome. Human brain endothelial cells (HBECs) are resistant to Escherichia coli-derived Shiga toxin (Stx); however, inflammatory cytokines markedly increase HBEC sensitivity to Stx cytotoxicity. HBECs were exposed to tumor necrosis factor (TNF)-alpha, with and without Stx-1, and cell survival, (125)I-Stx1 binding, globotriaosylceramide content, cell necrosis, and cell apoptosis levels were determined. TNF greatly increased Stx-1 cytotoxicity, primarily through induction of apoptosis, in HBEC.
Volume
187
Issue
1
First Page
154
Last Page
158
ISSN
0022-1899
Published In/Presented At
Ergonul, Z., Hughes, A. K., & Kohan, D. E. (2003). Induction of apoptosis of human brain microvascular endothelial cells by shiga toxin 1. The Journal of infectious diseases, 187(1), 154–158. https://doi.org/10.1086/345861
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
12508161
Department(s)
Department of Pediatrics
Document Type
Article