U-73122 does not specifically inhibit phospholipase C in rat pancreatic islets and insulin-secreting beta-cell lines.
Publication/Presentation Date
1-1-1994
Abstract
Phospholipase C is activated in insulin secretion by islets of Langerhans and insulin-secreting beta-cells such as RINm5F and beta-TC3. We have examined the effects of the aminosteroid U-73122, a phospholipase C inhibitor, on insulin secretion and phospholipase C activation. U-73122 slightly inhibited glucose-induced insulin secretion from islets, but this effect was not specific since the structural "inactive" analogue U-73343 also inhibited insulin secretion. Likewise, in RINm5F cells, U-73122 did not inhibit glyceraldehyde-induced insulin secretion. Phospholipase C activity was assessed as the accumulation of inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) measured with a competitive binding assay: U-73122 failed to inhibit glucose-induced increase in Ins(1,4,5)P3. Similarly, when the effects of U-73122 and U-73343 were measured on [3H]phosphatidylinositol hydrolysis of islets, both compounds caused a slight, non-specific inhibition of phospholipase C activity. These observations suggest that U-73122 does not specifically inhibit phospholipase C in insulin-secreting cells.
Volume
54
Issue
8
First Page
107
Last Page
112
ISSN
0024-3205
Published In/Presented At
Alter, C. A., Amagasu, M., Shah, K., Jolly, Y. C., Major, C., & Wolf, B. A. (1994). U-73122 does not specifically inhibit phospholipase C in rat pancreatic islets and insulin-secreting beta-cell lines. Life sciences, 54(8), PL107–PL112. https://doi.org/10.1016/0024-3205(94)90009-4
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
8107526
Department(s)
Department of Pediatrics
Document Type
Article