Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation.
Publication/Presentation Date
1-1-2014
Abstract
Patients with chronic kidney disease (CKD), who usually display low serum 25-hydroxyvitamin D (25D) and 1,25-dihydroxyvitamin D (1,25D), are at high risk of infection, notably those undergoing peritoneal dialysis (PD). We hypothesized that peritoneal macrophages from PD patients are an important target for vitamin D-induced antibacterial activity. Dialysate effluent fluid was obtained from 27 non-infected PD patients. Flow cytometry indicated that PD cells were mainly monocytic (37.9±17.7% cells CD14+/CD45+). Ex vivo analyses showed that PD cells treated with 25D (100 nM, 6 hrs) or 1,25D (5 nM, 6 hrs) induced mRNA for antibacterial cathelicidin (CAMP) but conversely suppressed mRNA for hepcidin (HAMP). PD cells from patients with peritonitis (n = 3) showed higher baseline expression of CAMP (18-fold±9, p
Volume
9
Issue
12
First Page
116530
Last Page
116530
ISSN
1932-6203
Published In/Presented At
Bacchetta, J., Chun, R. F., Gales, B., Zaritsky, J. J., Leroy, S., Wesseling-Perry, K., Boregaard, N., Rastogi, A., Salusky, I. B., & Hewison, M. (2014). Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation. PloS one, 9(12), e116530. https://doi.org/10.1371/journal.pone.0116530
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
25549329
Department(s)
Department of Pediatrics
Document Type
Article