Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation.
Publication/Presentation Date
12-1-2024
Abstract
OBJECTIVE: The objective of this study was to determine the frequency of common gastrointestinal bacterial, parasitic, and viral pathogen detection in necrotizing enterocolitis (NEC) or spontaneous intestinal perforation (SIP) -associated intestinal tissue.
STUDY DESIGN: Retrospective cohort study examined formalin fixed, paraffin embedded (FFPE) surgical or autopsy intestinal tissue from NEC or SIP specimens. DNA and RNA were extracted and analyzed by multiplex PCR panel (GIFA Biofire). DNA or RNA from stool samples containing each pathogen were extracted for positive controls.
RESULTS: The total number of intestinal tissue samples were 193 from 310 infants (156 NEC, 37 SIP). Six (3%) infants with stage III NEC tested positive for a target pathogen; 2, C. difficile; 3, Enteroaggregtive E. coli; and 1, Giardia. No gastrointestinal viral pathogens were detected.
CONCLUSION: Molecular testing yielded few GI pathogens suggesting that these organisms are likely not major causes or facilitators of NEC or SIP.
Volume
44
Issue
12
First Page
1755
Last Page
1761
ISSN
1476-5543
Published In/Presented At
Talavera-Barber, M. M., Sánchez, P. J., Conces, M., Kaptsan, I., Everhart, K., Leber, A., Malleske, D. T., Moallem, M., Panesso-Gómez, S., & Shimamura, M. (2024). Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation. Journal of perinatology : official journal of the California Perinatal Association, 44(12), 1755–1761. https://doi.org/10.1038/s41372-024-01999-z
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
38849546
Department(s)
Department of Pediatrics
Document Type
Article
