Hypoxia-induced Ca2+-influx in cerebral cortical neuronal nuclei of newborn piglets.
Publication/Presentation Date
5-15-2003
Abstract
The present study was designed to investigate the effect of hypoxia on nuclear calcium-influx in the cerebral cortex of newborn piglets. Anesthetized and ventilated newborn piglets divided into normoxic (n=4) and hypoxic groups with varying degrees of tissue hypoxia (n=10) were studied. Nuclear Ca(2+)-influx was determined using (45)Ca(2+) and plotted against ATP and phosphocreatine levels. The plots were analyzed by non-linear regression (exponential) analysis that showed a curvilinear relationship (r=0.92 for ATP and r=0.88 for phosphocreatine). These data suggest a threshold at which there is a sudden increase in the nuclear calcium-influx that then continues to increase with further decrease in the ATP and phosphocreatine levels. The results demonstrate an increase in the nuclear Ca(2+)-influx during hypoxia in newborn piglets and that this increase correlates in a curvilinear fashion with the increase in the degree of cerebral tissue hypoxia. We propose that the hypoxia-induced increase in intranuclear Ca(2+) is due to altered nuclear membrane Ca(2+)-influx mechanisms and will lead to Ca(2+)-mediated alteration of apoptotic gene expression as well as Ca(2+)-dependent activation of endonucleases that result in DNA fragmentation and subsequent programmed neuronal cell death.
Volume
342
Issue
1-2
First Page
119
Last Page
123
ISSN
0304-3940
Published In/Presented At
Delivoria-Papadopoulos, M., Akhter, W., & Mishra, O. P. (2003). Hypoxia-induced Ca2+-influx in cerebral cortical neuronal nuclei of newborn piglets. Neuroscience letters, 342(1-2), 119–123. https://doi.org/10.1016/s0304-3940(03)00256-8
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
12727332
Department(s)
Department of Pediatrics
Document Type
Article