Protein synthesis and degradation in skeletal muscle of chronically uremic rats.
Publication/Presentation Date
6-1-1986
Abstract
We used the perfused hemicorpus preparation to measure individual rates of protein synthesis and degradation. Using fed animals, perfused either with or without insulin, muscle protein synthesis and hemicorpus protein degradation rates were similar, but myofibrillar protein degradation was clearly increased in the uremic preparations. When the animals were fasted, differences in the rates of skeletal muscle protein turnover were apparent. Uremic rats lost more wt at both 24 and 48 hr of fasting when compared to either ad libitum fed or pair-fed controls who started fasting at body wts equivalent to our uremic rats. The accelerated wt loss was accompanied by lower rates of protein synthesis, higher degradation rates, and greater net protein catabolism in our uremic rats. Alterations in body lipid content were present in uremia and correlated with the rate of protein degradation in both control and uremic rats. These data demonstrated that even in the fed state, uremia is associated with subtle alterations in skeletal muscle protein turnover. When stressed, these alterations become more pronounced. Insufficient stores of body lipids, either due to inadequate nutrition or altered metabolism, may contribute to the alterations in muscle protein turnover seen in chronic renal insufficiency.
Volume
29
Issue
6
First Page
1136
Last Page
1143
ISSN
0085-2538
Published In/Presented At
Li, J. B., & Wassner, S. J. (1986). Protein synthesis and degradation in skeletal muscle of chronically uremic rats. Kidney international, 29(6), 1136–1143. https://doi.org/10.1038/ki.1986.119
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
3747330
Department(s)
Department of Pediatrics
Document Type
Article