Nitric oxide synthase inhibition with NG-mono-methyl-L-arginine reversibly decreases cerebral blood flow in piglets.
Publication/Presentation Date
3-1-1994
Abstract
OBJECTIVE: We tested the hypothesis that, in piglets, the intravenous administration of the reversible inhibitor of nitric oxide synthase, NG-mono-methyl-L-arginine, decreases cerebral blood flow via a mechanism unrelated to cerebral oxygen consumption.
DESIGN: Prospective, randomized, controlled animal study.
SETTING: Animal laboratory at a university.
SUBJECTS: Pentobarbital-anesthetized piglets (1 to 2 wks of age; 2.6 to 4.0 kg).
INTERVENTIONS: Piglets were treated with either 50 mg of NG-mono-methyl-L-arginine, 100 mg of NG-mono-methyl-L-arginine, or an equal volume of saline by intravenous infusion over 10 mins.
MEASUREMENTS AND MAIN RESULTS: Mean arterial pressure increased after NG-mono-methyl-L-arginine (50 mg dose: 84 +/- 6 to 100 +/- 7 mmHg; 100 mg dose: 82 +/- 4 to 107 +/- 4 mmHg; p < .001). Forebrain blood flow (microspheres) decreased (37 +/- 2 to 30 +/- 2 mL/min/100 g; p < .05) and cerebrovascular resistance increased (2.1 +/- 0.2 to 3.5 +/- 0.3 mmHg/mL/min/100 g; p < .05) only after 100 mg of NG-mono-methyl-L-arginine. Neurohypophysis blood flow decreased to 56 +/- 9% of the control value, while forebrain blood flow decreased only to 81 +/- 4% of the control value after 100 mg of NG-mono-methyl-L-arginine administration. Blood flow returned to control values by 30 mins after infusion. NG-mono-methyl-L-arginine administration had no effect on cerebral oxygen consumption at either dose. Intravenous administration of L-arginine (300 mg) immediately after the infusion of 100 mg of NG-mono-methyl-L-arginine was associated with prompt (by 3 mins) recovery of blood flow to all brain regions that were affected by NG-mono-methyl-L-arginine.
CONCLUSIONS: These data suggest that nitric oxide and/or a nitric oxide-containing substance is an important mediator of cerebrovascular tone in piglets, acting via a mechanism unrelated to altering cerebral oxygen consumption.
Volume
22
Issue
3
First Page
384
Last Page
392
ISSN
0090-3493
Published In/Presented At
Greenberg, R. S., Helfaer, M. A., Kirsch, J. R., Moore, L. E., & Traystman, R. J. (1994). Nitric oxide synthase inhibition with NG-mono-methyl-L-arginine reversibly decreases cerebral blood flow in piglets. Critical care medicine, 22(3), 384–392. https://doi.org/10.1097/00003246-199403000-00006
Disciplines
Medicine and Health Sciences | Pediatrics
PubMedID
7510221
Department(s)
Department of Pediatrics
Document Type
Article